| Official Full Name |
Recombinant Rat LPS-induced CXC Chemokine/CXCL5 (rRtLIX/CXCL5) |
| Squence |
 |
| Amino Acid Sequence |
APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ |
| Synonyms |
Cytokine LIX, Small-inducible Cytokine B5 |
| Accession Number |
P97885 |
| GeneID |
60665 |
| Summary |
CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. The expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. Recombinant rat CXCL5 contains 93 amino acids which is a single, non-glycosylated polypeptide chain. Rat CXCL5 shares 57 % amino acid sequence identity with human CXCL5. |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 10.1 kDa, a single, non-glycosylated polypeptide chain containing 93 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rRtLIX/CXCL5 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
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| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
