Recombinant Rat LPS-induced CXC Chemokine/CXCL5 (rRtLIX/CXCL5, P97885, 60665) CAS#: 241-05-1816

Official Full Name Recombinant Rat LPS-induced CXC Chemokine/CXCL5 (rRtLIX/CXCL5)
Squence Squence of Recombinant Rat LPS-induced CXC Chemokine/CXCL5
Amino Acid Sequence APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ
Synonyms Cytokine LIX, Small-inducible Cytokine B5
Accession Number P97885
GeneID 60665
Summary CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. The expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. Recombinant rat CXCL5 contains 93 amino acids which is a single, non-glycosylated polypeptide chain. Rat CXCL5 shares 57 % amino acid sequence identity with human CXCL5.
Source Escherichia coli.
Molecular Weight Approximately 10.1 kDa, a single, non-glycosylated polypeptide chain containing 93 amino acids.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rRtLIX/CXCL5 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Rat LPS-induced CXC Chemokine/CXCL5
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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