| Official Full Name |
Recombinant Rat Stem Cell Factor (rRtSCF) |
| Squence |
 |
| Amino Acid Sequence |
MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA |
| Synonyms |
Hematopoietic growth factor KL、MGF |
| Accession Number |
P21581 |
| GeneID |
60427 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 mast cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rRtSCF as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Ronnstrand L. 2004. Cell Mol Life Sci. 61:2535-48.2. Anderson DM, Williams DE, Tushinski R, et al. 1991. Cell Growth Differ. 2:373-8.3. Brannan CI, Lyman SD, Williams DE, et al. 1991. Proc Natl Acad Sci U S A. 88:4671-4.4. Okayama Y, Kawakami T. 2006. Immunol Res. 34:97-115. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
