Official Full Name |
Recombinant Rat Stromal-Cell Derived Factor-1 beta/CXCL12 beta (rRtSDF-1beta/CXCL12beta) |
Squence |
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Amino Acid Sequence |
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM |
Synonyms |
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Accession Number |
Q9QZD1 |
GeneID |
24772 |
Summary |
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Source |
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Molecular Weight |
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 100-200 ng/ml. |
Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
Endotoxin |
Less than 1 EU/ug of rRtSDF-1beta/CXCL12beta as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53. |
SDS-PAGE |
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Safety Data Sheet (SDS) Download |
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Technical Data Sheet (TDS) Download |
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