Recombinant Rat Vascular Endothelial Growth Factor 164 (rRtVEGF164, P16612, 83785) CAS#: 145-07-1816; ChemWhat Code: 1404312

Official Full Name Recombinant Rat Vascular Endothelial Growth Factor 164 (rRtVEGF164)
Squence Squence of Recombinant Rat Vascular Endothelial Growth Factor 164
Amino Acid Sequence MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Synonyms VPF
Accession Number P16612
GeneID 83785
Summary
Source Escherichia coli.
Molecular Weight Approximately 38.7 kDa, a disulfide-linked homodimeric protein, consisting of two 165 amino acid polypeptide chains with Met at N-terminus.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.8.
Endotoxin Less than 1 EU/ug of rRtVEGF164 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9.2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94.3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.
SDS-PAGE SDS-PAGE of Recombinant Rat Vascular Endothelial Growth Factor 164
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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