| Official Full Name |
Recombinant Staphylokinase (rSAK) |
| Squence |
 |
| Amino Acid Sequence |
SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDGKRNELL SPRYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK |
| Synonyms |
Sak42D |
| Accession Number |
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| GeneID |
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| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 15.6 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 5.0 × 104 IU/mg. |
| Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rSAK as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Matsuo O, Okada K, Fukao H, et al. 1990. Blood, 76: 925-9.2. Collen D, Schlott B, Engelborghs Y, et al. 1993. J Biol Chem, 268: 8284-9.3. Shishido Y, Matsumoto T, Sakai M, et al. 1994. Biol Pharm Bull, 17: 1060-4.4. Ohlenschlager O, Ramachandran R, Flemming J, et al. 1997. J Biomol NMR, 9: 273-86. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
