| Official Full Name |
Recombinant Viral Macrophage Inflammatory Protein-2 (rViMIP-2) |
| Squence |
 |
| Amino Acid Sequence |
LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA |
| Synonyms |
Viral Macrophage Inflammatory Protein II, vMIP-1B, MIP-II, vMIP-2 |
| Accession Number |
Q98157 |
| GeneID |
4961514 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 8.0 kDa, a single, non-glycosylated polypeptide chain containing 70 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The specific activity is determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0ug-10.0ug/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150mM NaCl. |
| Endotoxin |
Less than 1 EU/ug of rViMIP-2 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Liwang AC, Wang ZX, Sun Y, et al. 1999. Protein Sci, 8: 2270-80.2. Morris KV, Higgins J, Shen X, et al. 2003. Virus Res, 94: 103-12.3. Luttichau HR. 2008. Virol J, 5: 50.4. Shao W, Fernandez E, Sachpatzidis A, et al. 2001. Eur J Biochem, 268: 2948-59. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
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