Requires Ca2+. The enzyme from?Thalassomonas?sp. can use agarose, agarohexaose and neoagarohexaose as substrate. The products of agarohexaose hydrolysis are dimers and tetramers, with agarotetraose being the predominant product, whereas hydrolysis of neoagarohexaose gives rise to two types of trimer. While the enzyme can also hydrolyse the highly sulfated agarose porphyran very efficiently, it cannot hydrolyse the related compounds ¦Ê-carrageenan (see EC?3.2.1.83) and ¦É-carrageenan (see EC?3.2.1.157) [2]. See also EC?3.2.1.81, ¦Â-agarase.
Cofactor
Ca2+
History
Reactions
Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product.
