bacterial luciferase EC#: 1.14.14.3; ChemWhat Code: 1375495
| Product Name | bacterial luciferase |
| Example Structure |  |
| Synonyms | 4a-hydroperoxy-4a,5-dihydroFMN intermediate luciferase, aldehyde monooxygenase, alkanal monooxygenase (FMN), bacterial luciferase, COB, Gluc luciferase, HFOOH, luciferase, Lux, LuxA, LuxAB, LuxB, LuxCDABE, LuxF, Vibrio fischeri luciferase, Vibrio harveyi luciferase |
| EC Number | 1.14.14.3 |
| CAS Registry Number | 9014-00-0 |
| Comments | The reaction sequence starts with the incorporation of a molecule of oxygen into reduced FMN bound to the enzyme, forming luciferase peroxyflavin. The peroxyflavin interacts with an aliphatic long-chain aldehyde, producing a highly fluorescent species believed to be luciferase hydroxyflavin. The enzyme is highly specific for reduced FMN and for long-chain aliphatic aldehydes with eight carbons or more. The highest efficiency is achieved with tetradecanal. cf. EC 1.13.12.18, dinoflagellate luciferase. |
| Cofactor | |
| History | |
| Reactions | A long-chain aldehyde + FMNH(2) + O(2) = a long-chain fatty acid + fmn + H(2)O + light. |
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