Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein, Accession#: A0A2K5V2X4, CAS#: 3202-706-251; ChemWhat Code: 1412219
Overview
| Product Description | Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-terminal.It contains Thr141-Leu285. |
| Biotinylated | Yes |
Specification
| Target | BAFF/TNFSF13B/CD257 Trimer |
| Synonyms | BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
| Gene/Protein ID (Accession) | A0A2K5V2X4 |
| Species | Cynomolgus |
| Exact Sequence | Thr141-Leu285 |
| Molecular Weight | The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi,N-Flag |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412219 | > 95% as determined by Tris-Bis PAGE |
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