Biotinylated Cynomolgus PD-L1/B7-H1 Protein (Primary Amine Labeling), Accession#: G7PSE7, CAS#: 3202-706-465; ChemWhat Code: 1412533

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Cynomolgus PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminal.It contains Phe19-Arg238.
Biotinylated Yes

Specification

Target PD-L1/B7-H1
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Gene/Protein ID (Accession) G7PSE7
Species Cynomolgus
Exact Sequence Phe19-Arg238
Molecular Weight The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Cynomolgus PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22um filtered solution in PBS (pH 7.4).
Reconstitution
Stability and Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412533> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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