Biotinylated Cynomolgus Siglec-10 Protein (Primary Amine Labeling), Accession#: A0A2K5WBX8, CAS#: 3202-706-220; ChemWhat Code: 1412171

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Cynomolgus Siglec-10 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Thr17-Asn552.
Biotinylated Yes

Specification

Target Siglec-10
Synonyms
Gene/Protein ID (Accession) A0A2K5WBX8
Species Cynomolgus
Exact Sequence Thr17-Asn552
Molecular Weight The protein has a predicted MW of 59.90 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Cynomolgus Siglec-10, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22um filtered solution in 25mM MES, 150mM NaCl, 0.5M Arginine (pH 5.0).
Reconstitution
Stability and Storage Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412171> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
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