Biotinylated Cynomolgus TIGIT Protein, Accession#: XP_015300911, CAS#: 3202-706-489; ChemWhat Code: 1412578
Overview
| Product Description | Recombinant Biotinylated Cynomolgus TIGIT Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Met89-Ile208. |
| Biotinylated | Yes |
Specification
| Target | TIGIT |
| Synonyms | TIGIT; VSIG9; VSTM3; WUCAM |
| Gene/Protein ID (Accession) | XP_015300911 |
| Species | Cynomolgus |
| Exact Sequence | Met89-Ile208 |
| Molecular Weight | The protein has a predicted MW of 16.2 kDa. Due to glycosylation, the protein migrates to 20-28 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Cynomolgus TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 59.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412578 | > 95% as determined by Tris-Bis PAGE |
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