Biotinylated Human ANGPT2/Angiopoietin-2 Protein, Accession#: O15123, CAS#: 3202-706-1460; ChemWhat Code: 1413965
Overview
| Product Description | Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Lys275-Phe496. |
| Biotinylated | Yes |
Specification
| Target | ANGPT2/Angiopoietin-2 |
| Synonyms | AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand |
| Gene/Protein ID (Accession) | O15123 |
| Species | Human |
| Exact Sequence | Lys275-Phe496 |
| Molecular Weight | The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-ANGPT2 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPT2, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413965 | > 95% as determined by Tris-Bis PAGE |
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