Biotinylated Human APRIL/TNFSF13 Trimer Protein (Primary Amine Labeling), Accession#: O75888, CAS#: 3202-706-639; ChemWhat Code: 1412794
Overview
Product Description | Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-terminal.It contains Lys112-Leu250. |
Biotinylated | Yes |
Specification
Target | APRIL/TNFSF13 Trimer |
Synonyms | |
Gene/Protein ID (Accession) | O75888 |
Species | Human |
Exact Sequence | Lys112-Leu250 |
Molecular Weight | The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag | N-His-Flag |
Express System | HEK293 |
Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
Form | Liquid |
Shipping | Shipped with blue ice. |
Formulation | Supplied as 0.22um filtered solution in PBS, 200mM Arginine (pH 7.4). |
Reconstitution | |
Stability and Storage | Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles. |
Validation Spectrums
Spectrums | |
Buy Reagent | |
ChemWhat 1412794 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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