Biotinylated Human CD40/TNFRSF5 Protein, Accession#: P25942, CAS#: 3202-706-1349; ChemWhat Code: 1413837
Overview
| Product Description | Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu21-Arg193. |
| Biotinylated | Yes |
Specification
| Target | CD40/TNFRSF5 |
| Synonyms | CD40; CD40L receptor; TNFRSF5;B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform;CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 |
| Gene/Protein ID (Accession) | P25942 |
| Species | Human |
| Exact Sequence | Glu21-Arg193 |
| Molecular Weight | The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413837 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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