Biotinylated Human FAP Protein, Accession#: Q12884, CAS#: 3202-706-372; ChemWhat Code: 1412361
Overview
| Product Description | Recombinant Biotinylated Human FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Leu26-Asp760. |
| Biotinylated | Yes |
Specification
| Target | FAP |
| Synonyms | FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
| Gene/Protein ID (Accession) | Q12884 |
| Species | Human |
| Exact Sequence | Leu26-Asp760 |
| Molecular Weight | The protein has a predicted MW of 87.9 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-FAP Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FAP, His Tag with the EC50 of 55.6ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM Tirs, 150mM NaCl, 200mM L-Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412361 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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