Biotinylated Human FGL1 Protein (Primary Amine Labeling), Accession#: Q08830, CAS#: 3202-706-593; ChemWhat Code: 1412734

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Human FGL1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminal.It contains Asp64-Asn305.
Biotinylated Yes

Specification

Target FGL1
Synonyms
Gene/Protein ID (Accession) Q08830
Species Human
Exact Sequence Asp64-Asn305
Molecular Weight The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Product Tag N-hFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4).
Reconstitution
Stability and Storage Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412734> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
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