Biotinylated Human GDF15 Protein, Accession#: Q99988, CAS#: 3202-706-527; ChemWhat Code: 1412648
Overview
| Product Description | Recombinant Biotinylated Human GDF15 Protein is expressed from E.coli with His tag and Avi tag at the N-terminal.It contains Ala197-Ile308. |
| Biotinylated | Yes |
Specification
| Target | Biotinylated GDF15 |
| Synonyms | GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta |
| Gene/Protein ID (Accession) | Q99988 |
| Species | Human |
| Exact Sequence | Ala197-Ile308 |
| Molecular Weight | The protein has a predicted MW of 15.19 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human GFRAL, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 17.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412648 | > 95% as determined by Tris-Bis PAGE |
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