Biotinylated Human IGF1R/CD221 Protein, Accession#: P08069, CAS#: 3202-706-721; ChemWhat Code: 1412932
Overview
| Product Description | Recombinant Biotinylated Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu31-Asn932. |
| Biotinylated | Yes |
Specification
| Target | IGF1R/CD221 |
| Synonyms | CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216 |
| Gene/Protein ID (Accession) | P08069 |
| Species | Human |
| Exact Sequence | Glu31-Asn932 |
| Molecular Weight | The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-IGF1R Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412932 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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