Biotinylated Human IL-17A/CTLA-8 Protein, Accession#: Q16552, CAS#: 3202-706-1189; ChemWhat Code: 1413653
Overview
| Product Description | Recombinant Biotinylated Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly24-Ala155. |
| Biotinylated | Yes |
Specification
| Target | IL-17A/CTLA-8 |
| Synonyms | CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A |
| Gene/Protein ID (Accession) | Q16552 |
| Species | Human |
| Exact Sequence | Gly24-Ala155 |
| Molecular Weight | The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test). The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413653 | > 95% as determined by Tris-Bis PAGE |
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