Biotinylated Human IL-2 R alpha/CD25 Protein, Accession#: P01589, CAS#: 3202-706-843; ChemWhat Code: 1413133
Overview
| Product Description | Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu22-Cys213. |
| Biotinylated | Yes |
Specification
| Target | IL-2 R alpha/CD25 |
| Synonyms | CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR |
| Gene/Protein ID (Accession) | P01589 |
| Species | Human |
| Exact Sequence | Glu22-Cys213 |
| Molecular Weight | The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413133 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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