Biotinylated Human IL-22R alpha 1&IL-10R beta Protein, Accession#: Q8N6P7, CAS#: 3202-706-520; ChemWhat Code: 1412636
Overview
| Product Description | Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-terminal.It contains His16-Thr228(IL-22R alpha 1)&Met20-Ser220(IL-10R beta). |
| Biotinylated | Yes |
Specification
| Target | IL-22R alpha 1&IL-10R beta |
| Synonyms | |
| Gene/Protein ID (Accession) | Q8N6P7 |
| Species | Human |
| Exact Sequence | His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta) |
| Molecular Weight | The protein has a predicted MW of 52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412636 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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