Biotinylated Human IL-25/IL-17E Protein, Accession#: Q9H293, CAS#: 3202-706-393; ChemWhat Code: 1412403
Overview
Product Description | Recombinant Biotinylated Human IL-25/IL-17E Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Tyr33-Gly177. |
Biotinylated | Yes |
Specification
Target | IL-25/IL-17E |
Synonyms | IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25 |
Gene/Protein ID (Accession) | Q9H293 |
Species | Human |
Exact Sequence | Tyr33-Gly177 |
Molecular Weight | The protein has a predicted MW of 19.24 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | HEK293 |
Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
Form | Liquid |
Shipping | Shipped with blue ice. |
Formulation | Supplied as 0.22um filtered solution in PBS (pH 7.4). |
Reconstitution | |
Stability and Storage | Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles. |
Validation Spectrums
Spectrums | |
Buy Reagent | |
ChemWhat 1412403 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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