Biotinylated Human IL-4 Protein, Accession#: P05112, CAS#: 3202-706-1128; ChemWhat Code: 1413573
Overview
| Product Description | Recombinant Biotinylated Human IL-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains His25-Ser153. |
| Biotinylated | Yes |
Specification
| Target | IL-4 |
| Synonyms | IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1 |
| Gene/Protein ID (Accession) | P05112 |
| Species | Human |
| Exact Sequence | His25-Ser153 |
| Molecular Weight | The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-4 R alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413573 | > 95% as determined by Tris-Bis PAGE |
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