Biotinylated Human LAG3/CD223 Protein, Accession#: P18627, CAS#: 3202-706-1034; ChemWhat Code: 1413443
Overview
| Product Description | Recombinant Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Leu23-Gly434. |
| Biotinylated | Yes |
Specification
| Target | LAG3/CD223 |
| Synonyms | CD223; FDC; LAG3; LAG-3; sLAG-3 |
| Gene/Protein ID (Accession) | P18627 |
| Species | Human |
| Exact Sequence | Leu23-Gly434 |
| Molecular Weight | The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-LAG3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413443 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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Other Suppliers | |
| Watson International Limited | Visit Watson Official Website |
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