Biotinylated Human Mature TGF beta 1 Protein, Accession#: P01137, CAS#: 3202-706-488; ChemWhat Code: 1412576
Overview
| Product Description | Recombinant Biotinylated Human Mature TGF beta 1 Protein is expressed from HEK293 with Avi tag at the C-terminal.It contains Ala279-Ser390. |
| Biotinylated | Yes |
Specification
| Target | Mature TGF beta 1 |
| Synonyms | CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1 |
| Gene/Protein ID (Accession) | P01137 |
| Species | Human |
| Exact Sequence | Ala279-Ser390 |
| Molecular Weight | The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition and 26-30 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Activity | Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM Glycine, 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine, 150mM NaCl (pH 2.5). |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412576 | > 95% as determined by Tris-Bis PAGE |
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