Biotinylated Human Mature TGF beta 2 Protein, Accession#: P61812, CAS#: 3202-706-1144; ChemWhat Code: 1413591

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed from HEK293 with Avi tag at the C-terminal.It contains Ala303-Ser414.
Biotinylated Yes

Specification

Target Mature TGF beta 2
Synonyms
Gene/Protein ID (Accession) P61812
Species Human
Exact Sequence Ala303-Ser414
Molecular Weight The protein has a predicted MW of 14.53 kDa same as Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.27ug/ml determined by ELISA. See testing image for detail.
Product Tag C-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413591> 95% as determined by Tris-Bis PAGE
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