Biotinylated Human SOST/Sclerostin Protein, Accession#: Q9BQB4, CAS#: 3202-706-1109; ChemWhat Code: 1413545
Overview
| Product Description | Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-terminal.It contains Gln24-Tyr213. |
| Biotinylated | Yes |
Specification
| Target | SOST/Sclerostin |
| Synonyms | sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH |
| Gene/Protein ID (Accession) | Q9BQB4 |
| Species | Human |
| Exact Sequence | Gln24-Tyr213 |
| Molecular Weight | The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-SOST Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413545 | > 95% as determined by Tris-Bis PAGE |
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