Biotinylated Mouse GDF15 Protein (Primary Amine Labeling), Accession#: Q9Z0J7, CAS#: 3202-706-1667; ChemWhat Code: 1414183
Overview
| Product Description | Recombinant Biotinylated Mouse GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminal.It contains Ser189-Ala303. |
| Biotinylated | Yes |
Specification
| Target | GDF15 |
| Synonyms | GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta |
| Gene/Protein ID (Accession) | Q9Z0J7 |
| Species | Mouse |
| Exact Sequence | Ser189-Ala303 |
| Molecular Weight | The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Mouse GFRAL, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 10ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414183 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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