Biotinylated Mouse IL-17A&F Protein, Accession#: Q62386, CAS#: 3202-706-754; ChemWhat Code: 1412979
Overview
| Product Description | Recombinant Biotinylated Mouse IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Ala26-Ala158(IL-17A)&Arg29-Ala161(IL-17F). |
| Biotinylated | Yes |
Specification
| Target | IL-17A&F |
| Synonyms | IL-17A；Interleukin-17A；CTLA-8；IL-17；IL-17F；IL24；ML-1；Interleukin-17F |
| Gene/Protein ID (Accession) | Q62386 |
| Species | Mouse |
| Exact Sequence | Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F) |
| Molecular Weight | The protein has a predicted MW of 17.9 kDa(IL-17A)&14.9 kDa(IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa&27-30 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Mouse IL-17R alpha, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A&F, His Tag with the EC50 of 19.6ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412979 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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