Biotinylated Mouse OX40/TNFRSF4/CD134 Protein, Accession#: P47741, CAS#: 3202-706-872; ChemWhat Code: 1413187
Overview
| Product Description | Recombinant Biotinylated Mouse OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Val20-Pro211. |
| Biotinylated | Yes |
Specification
| Target | OX40/TNFRSF4/CD134 |
| Synonyms | CD134 antigen; CD134;OX40; OX40L receptor; TNFRSF4;ACT-135; ACT35 antigen; ACT35ATC35 antigen; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen |
| Gene/Protein ID (Accession) | P47741 |
| Species | Mouse |
| Exact Sequence | Val20-Pro211 |
| Molecular Weight | The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Mouse OX40 Ligand, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 4.3ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413187 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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