Biotinylated Rat MASP2 Protein (Primary Amine Labeling), Accession#: A2VCV7, CAS#: 3202-706-454; ChemWhat Code: 1412513
Overview
| Product Description | Recombinant Biotinylated Rat MASP2 Protein (Primary Amine Labeling) is expressed from E.coli with His tag at the C-terminal.It contains Thr287-Phe685. |
| Biotinylated | Yes |
Specification
| Target | MASP2 |
| Synonyms | MAP19; MASP-2; MASP1P1; sMap |
| Gene/Protein ID (Accession) | A2VCV7 |
| Species | Rat |
| Exact Sequence | Thr287-Phe685 |
| Molecular Weight | The protein has a predicted MW of 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-MASP2 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Rat MASP2, His Tag with the EC50 of 19.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Formulation | Supplied as 0.22um filtered solution in 50mM Tris, 200mM NaCl (pH 9.0). |
| Reconstitution | |
| Stability and Storage | Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412513 | > 95% as determined by Tris-Bis PAGE |
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