The enzyme appears to work equally well with both diastereoisomers of cholesterol 5,6-epoxide [3]. The product is a competitive inhibitor of the reaction. In vertebrates, five epoxide-hydrolase enzymes have been identified to date: EC?3.3.2.6?(leukotriene-A4?hydrolase), EC?3.3.2.7?(hepoxilin-epoxide hydrolase), EC?3.3.2.9?(microsomal epoxide hydrolase), EC?3.3.2.10?(soluble epoxide hydrolase) and EC?3.3.2.11?(cholesterol 5,6-oxide hydrolase) [3].
