Cynomolgus CD27 Ligand/CD70 Protein, Accession#: G7PYU6, CAS#: 3202-706-1702; ChemWhat Code: 1414202

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Cynomolgus CD27 Ligand/CD70 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Gln39-Pro194.
Biotinylated

Specification

Target CD27 Ligand/CD70
Synonyms CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG; Ki-24 antigen; TNFSF7G
Gene/Protein ID (Accession) G7PYU6
Species Cynomolgus
Exact Sequence Gln39-Pro194
Molecular Weight The protein has a predicted MW of 18.4 kDa. Due to glycosylation, the protein migrates to 60-90 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD27 Ligand, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 70.8ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1414202> 95% as determined by Tris-Bis PAGE;
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