Cynomolgus IL-12 Protein, Accession#: G7P6S2, CAS#: 3202-706-591; ChemWhat Code: 1412730
Overview
| Product Description | Recombinant Cynomolgus IL-12 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ile23-Ser328 (IL-12B) & Arg57-Ser253 (IL-12A). |
| Biotinylated |
Specification
| Target | IL-12 |
| Synonyms | |
| Gene/Protein ID (Accession) | G7P6S2 |
| Species | Cynomolgus |
| Exact Sequence | Ile23-Ser328(IL-12B) & Arg57-Ser253(IL-12A) |
| Molecular Weight | The protein has a predicted MW of 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412730 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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