Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein, Accession#: A0A2K5TLV4, CAS#: 3202-706-285; ChemWhat Code: 1412253
Overview
| Product Description | Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag at the N-terminal.It contains Val22-Pro189(Cynomolgus IL-23 alpha)&Met23-Ser335(Mouse IL-12 beta). |
| Biotinylated |
Specification
| Target | IL-23 alpha&IL-12 beta |
| Synonyms | IL23 alpha; IL12 beta; IL23 alpha&IL12 beta |
| Gene/Protein ID (Accession) | A0A2K5TLV4 |
| Species | Cynomolgus&Mouse |
| Exact Sequence | Val22-Pro189(Cynomolgus IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta) |
| Molecular Weight | The protein has a predicted MW of 19.5 (Cynomolgus IL23 alpha)&35.8 (Mouse IL12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 3.90 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412253 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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