Cynomolgus MASP2 Protein, Accession#: A0A2K5UJY0, CAS#: 3202-706-258; ChemWhat Code: 1412227
Overview
| Product Description | Recombinant Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-terminal.It contains Thr287-Phe686. |
| Biotinylated |
Specification
| Target | MASP2 |
| Synonyms | MAP19; MASP-2; MASP1P1; sMap |
| Gene/Protein ID (Accession) | A0A2K5UJY0 |
| Species | Cynomolgus |
| Exact Sequence | Thr287-Phe686 |
| Molecular Weight | The protein has a predicted MW of 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412227 | > 95% as determined by Tris-Bis PAGE |
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