Cynomolgus PCSK9 Protein, Accession#: A0A2K5TZC4, CAS#: 3202-706-179; ChemWhat Code: 1412127
Overview
| Product Description | Recombinant Cynomolgus PCSK9 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Glu151-Gln811. |
| Biotinylated |
Specification
| Target | PCSK9 |
| Synonyms | |
| Gene/Protein ID (Accession) | A0A2K5TZC4 |
| Species | Cynomolgus |
| Exact Sequence | Glu151-Gln811 |
| Molecular Weight | Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-70 kDa (pro-form) and 15-20 kDa (mature form) based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 81.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412127 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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