Cynomolgus Semaphorin 4D/SEMA4D/CD100 Protein, Accession#: A0A2K5TZC9, CAS#: 3202-706-238; ChemWhat Code: 1412203
Overview
| Product Description | Recombinant Cynomolgus Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Phe24-Arg734. |
| Biotinylated |
Specification
| Target | Semaphorin 4D/SEMA4D/CD100 |
| Synonyms | Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4 |
| Gene/Protein ID (Accession) | A0A2K5TZC9 |
| Species | Cynomolgus |
| Exact Sequence | Phe24-Arg734 |
| Molecular Weight | The protein has a predicted MW of 80.1 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus Semaphorin 4D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412203 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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