Cynomolgus TPBG/5T4 Protein, Accession#: Q4R8Y9, CAS#: 3202-706-1446; ChemWhat Code: 1413953

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Cynomolgus TPBG/5T4 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Thr35-Ser355.
Biotinylated

Specification

Target TPBG/5T4
Synonyms
Gene/Protein ID (Accession) Q4R8Y9
Species Cynomolgus
Exact Sequence Thr35-Ser355
Molecular Weight The protein has a predicted MW of 35.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413953> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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