The bifunctional protein?hldE?includes?D-glycero-¦Â-D-manno-heptose-7-phosphate kinase and?D-glycero-¦Â-D-manno-heptose 1-phosphate adenylyltransferase activity (cf. EC?2.7.7.70). The enzyme is involved in biosynthesis of ADP-L-glycero-¦Â-D-manno-heptose, which is utilized for assembly of the lipopolysaccharide inner core in Gram-negative bacteria. The enzyme selectively produces?D-glycero-¦Â-D-manno-heptose 1,7-bisphosphate [5].
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D-glycero-beta-D-manno-heptose 7-phosphate + Atp = D-glycero-beta-D- manno-heptose 1,7-bisphosphate + Adp.