DNA ligase (ATP or NAD+) EC#: 6.5.1.6; ChemWhat Code: 1383898
| Product Name | DNA ligase (ATP or NAD+) |
| Example Structure |  |
| Synonyms | Tfu DNA ligase |
| EC Number | 6.5.1.6 |
| CAS Registry Number | |
| Comments | The enzymes from the archaea?Thermococcus fumicolans?and?Thermococcus onnurineus?show high activity with either ATP or NAD+, and significantly lower activity with TTP, GTP, and CTP. The enzyme catalyses the ligation of DNA strands with 3′-hydroxyl and 5′-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in duplex DNA. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP or NAD+, forming a phosphoramide bond between adenylate and a lysine residue. The adenylate group is then transferred to the 5′-phosphate terminus of the substrate, forming the capped structure 5′-(5′-diphosphoadenosine)-[DNA]. Finally, the enzyme catalyses a nucleophilic attack of the 3′-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the adenylate. Different from EC?6.5.1.1, DNA ligase (ATP), EC?6.5.1.2, DNA ligase (NAD+) and EC?6.5.1.7, DNA ligase (ATP, ADP or GTP). |
| Cofactor | |
| History | |
| Reactions | (1) Atp + (deoxyribonucleotide)(n)-3′-hydroxyl + 5′-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + Amp + diphosphate. (2) NAD(+) + (deoxyribonucleotide)(n)-3′-hydroxyl + 5′-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + Amp + beta- nicotinamide D-nucleotide. |
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