GDP-4-dehydro-6-deoxy-alpha-D-mannose 3-dehydratase EC#: 4.2.1.168; ChemWhat Code: 1382486
| Product Name | GDP-4-dehydro-6-deoxy-alpha-D-mannose 3-dehydratase |
| Example Structure |  |
| Synonyms | colD, GDP-4-keto-6-deoxy-D-mannose-3-dehydratase, WbdK |
| EC Number | 4.2.1.168 |
| CAS Registry Number | |
| Comments | This enzyme, involved in ¦Â-L-colitose biosynthesis, is a unique vitamin-B6-dependent enzyme. In the first step of catalysis, the bound pyridoxal phosphate (PLP) cafactor is transaminated to the pyridoxamine 5′-phosphate (PMP) form of vitamin B6, using?L-glutamate as the amino group donor. The PMP cofactor then forms a Schiff base with the sugar substrate and the resulting adduct undergoes a 1,4-dehydration to eliminate the 3-OH group. Hydrolysis of the product from the enzyme restores the PLP cofactor and results in the release of an unstable enamine intermediate. This intermediate tautomerizes to form an imine form, which hydrolyses spontaneously, releasing ammonia and forming the final product. |
| Cofactor | |
| History | |
| Reactions | GDP-4-dehydro-alpha-D-rhamnose + L-glutamate = GDP-4-dehydro-3,6-dideoxy- alpha-D-mannose + 2-oxoglutarate + ammonia. |
Buy Reagent | |
| No reagent supplier? | Send quick inquiry to ChemWhat |
| Want to be listed here as a reagent supplier? (Paid service) | Click here to contact ChemWhat |
Approved Manufacturers | |
| Want to be listed as an approved manufacturer (Requires approvement)? | Please download and fill out this form and send back to approved-manufacturers@chemwhat.com |
Contact Us for Other Help | |
| Contact us for other information or services | Click here to contact ChemWhat |