Alg2, Alg2 mannosyltransferase, alpha-1,3-mannosyltransferase, asparagine-linked glycosylation 2, GDP-Man:Dol-PP-GlcNAc2Man2 alpha-1,3-mannosyltransferase, GDP-Man:Man1GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-oligosaccharide-lipid mannosyltransferase II, hALG2, mannosyltransferase II, mannosyltransferase, guanosine diphosphomannose-oligosaccharide-lipid II
EC Number
2.4.1.132
CAS Registry Number
81181-76-2
Comments
The biosynthesis of asparagine-linked glycoproteins utilizes a dolichyl diphosphate-linked glycosyl donor, which is assembled by the series of membrane-bound glycosyltransferases that comprise the dolichol pathway. Alg2 mannosyltransferase from Saccharomyces cerevisiae carries out an ¦Á1,3-mannosylation of D-Man-¦Â-(1¡ú4)-D-GlcNAc-¦Â-(1¡ú4)-D-GlcNAc-diphosphodolichol, followed by an ¦Á1,6-mannosylation (cf. EC 2.4.1.257), to form the first branched pentasaccharide intermediate of the dolichol pathway [1,2].
Cofactor
History
Reactions
GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc- diphosphodolichol = Gdp + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc- beta-(1->4)-GlcNAc-diphosphodolichol.
