Human ARTN Protein, Accession#: Q5T4W7, CAS#: 3202-706-981; ChemWhat Code: 1413359
Overview
| Product Description | Recombinant Human ARTN Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Ala108-Gly220. |
| Biotinylated |
Specification
| Target | ARTN |
| Synonyms | Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN |
| Gene/Protein ID (Accession) | Q5T4W7 |
| Species | Human |
| Exact Sequence | Ala108-Gly220 |
| Molecular Weight | The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 4mM HCl (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH2.8). |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413359 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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