Human BAFF/TNFSF13B/CD257 Protein, Accession#: Q9Y275, CAS#: 3202-706-1133; ChemWhat Code: 1413577
Overview
| Product Description | Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Ala134-Leu285. |
| Biotinylated |
Specification
| Target | BAFF/TNFSF13B/CD257 |
| Synonyms | BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
| Gene/Protein ID (Accession) | Q9Y275 |
| Species | Human |
| Exact Sequence | Ala134-Leu285 |
| Molecular Weight | The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-monomeric hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413577 | > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
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