Human BDCA-2 Protein, Accession#: Q8WTT0, CAS#: 3202-706-406; ChemWhat Code: 1412429
Overview
| Product Description | Recombinant Human BDCA-2 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Phe46-Ile213. |
| Biotinylated |
Specification
| Target | BDCA-2 |
| Synonyms | BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303; BDCA2MGC125791; CD303; CLECSF11PRO34150; DLEC; DLECMGC125792; HECLMGC125793; MGC125789; PRO34150 |
| Gene/Protein ID (Accession) | Q8WTT0 |
| Species | Human |
| Exact Sequence | Phe46-Ile213 |
| Molecular Weight | The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | Expi293 |
| Endotoxin | Less than 1EU per μg by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 200mM L-Arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412429 | > 95% as determined by Tris-Bis PAGE |
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