Human CA9/Carbonic Anhydrase IX Protein, Accession#: Q16790, CAS#: 3202-706-1645; ChemWhat Code: 1414159
Overview
| Product Description | Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln38-Asp414. |
| Biotinylated |
Specification
| Target | CA9/Carbonic Anhydrase IX |
| Synonyms | CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC |
| Gene/Protein ID (Accession) | Q16790 |
| Species | Human |
| Exact Sequence | Gln38-Asp414 |
| Molecular Weight | The protein has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CA9, His Tag at 0.9ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414159 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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