Human CCL5 Protein, Accession#: P13501, CAS#: 3202-706-1532; ChemWhat Code: 1414045
Overview
| Product Description | Recombinant Human CCL5 Protein is expressed from E.coli with His-SUMO tag at the N-terminal.It contains Ser24-Ser91. |
| Biotinylated |
Specification
| Target | CCL5 |
| Synonyms | MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5 |
| Gene/Protein ID (Accession) | P13501 |
| Species | Human |
| Exact Sequence | Ser24-Ser91 |
| Molecular Weight | The protein has a predicted MW of 21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
| Product Tag | N-His,SUMO |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM Tris,0.3M NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414045 | > 95% as determined by Tris-Bis PAGE |
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